To find the sex construction of Serbian populace try we used the CNVkit 0

To find the sex construction of Serbian populace try we used the CNVkit 0

Germline SNP and you can Indel variant contacting try did pursuing the Genome Studies Toolkit (GATK, v4.step one.0.0) most useful practice guidance sixty . Intense reads were mapped to the UCSC people source genome hg38 playing with a great Burrows-Wheeler Aligner (BWA-MEM, v0.eight.17) 61 . Optical and you can PCR duplicate establishing and sorting was over having fun with Picard (v4.step one.0.0) ( Feet high quality rating recalibration is completed with brand new GATK BaseRecalibrator resulting inside a final BAM declare for each test. The site documents utilized for foot high quality get recalibration was indeed dbSNP138, Mills and you can 1000 genome gold standard indels and you may 1000 genome stage step one, given on the GATK Resource Bundle (history changed 8/). (more…)

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